tissue culture and agrobacterium-mediated transformation of hemp from marijuana
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tissue culture and agrobacterium-mediated transformation of hemp from marijuana
Tissue Culture and Agrobacterium-Mediated …- tissue culture and agrobacterium-mediated transformation of hemp from marijuana ,TISSUE CULTURE AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF HEMP (CANNABIS SATIVA L.) M. FEENEY AND Z. K. PUNJA* Centre for Environmental Biology, Department of Biological Sciences, 8888 University Drive, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6 (Received 20 August 2002; accepted 30 April 2003; editor …How to Tissue Culture Cannabis - Plant Cell Technology ...23/02/2021·The tissue culture of Cannabis is the clonal propagation of the plant under a controlled aseptic environment. The technique is used to obtain secondary compounds from the plants, which mainly include cannabinoids. The techniques that have been extensively studied in terms of Cannabis tissue culture include callus induction and Agrobacterium-mediated …
Advances and Perspectives in Tissue Culture and Genetic ...
26/05/2021·2. In vitro Culture in Cannabis. In vitro culture is the basis of most biotechnological tools [10,47].Many methods such as micropropagation, in situ and ex situ conservation, cell culture, Agrobacterium-mediated gene transformation, and polyploidy induction completely depend on in vitro culture techniques [].Moreover, plant cell and tissue culture is also a …
Highly efficient, genotype‐independent transformation and ...
04/12/2021·Efficient genetic transformation has the potential to advance research and breeding in watermelon (Citrullus lanatus), but regeneration from tissue culture remains challenging.Previous work showed that expressing a fusion of two interacting transcription factors, GROWTH-REGULATING FACTOR4 (GRF4) and GRF-INTERACTING FACTOR1 (GIF1), …
tissue culture and agrobacterium-mediated transformation ...
Tissue culture and Agrobacterium-mediated transformation ... Hemp (Cannabis sativa L.) is cultivated in many parts of the world for ils fiber, oil, and seed. The development of new hemp cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to investigate the ...
Hemp (Cannabis sativa L.) | Springer Nature Experiments
Feeney M, Punja ZK (2003) Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.). In Vitro Cell Dev Biol Plant …
Hemp ( Cannabis sativa L.) | SpringerLink
20/09/2014·Agrobacterium-mediated transformation Callus Cannabis sativa Chlorophenol red assay Hemp Mannose selection Phosphomannose isomerase Plant tissue culture Suspension culture Transformation protocol This is a …
Establishment of an Agrobacterium‐mediated genetic ...
07/05/2021·Agrobacterium-mediated plant transformation. Agrobacterium strain containing the DR-modified vector was used to infect the explants. Single colony of AGL1 was selected and grown in YEP liquid media containing 50 mg/L kanamycin and 20 mg/L rifampicin in an incubator shaker 200 rpm overnight at 28°C. A. tumefaciens culture was centrifuged at 3500
Tissue culture and Agrobacterium-mediated transformation ...
Hemp (Cannabis sativa L.) is cultivated in many parts of the world for ils fiber, oil, and seed. The development of new hemp cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to investigate the propagation of hemp in tissue culture and to establish a protocol for Agrobacterium …
OPTIMIZATION OF TISSUE CULTURE, REGENERATION AND MEDIATED …
OPTIMIZATION OF TISSUE CULTURE, REGENERATION AND AGROBACTERIUM MEDIATED TRANSFORMATION PARAMATERS IN WINTER WHEAT CULTIVARS (Kızıltan-91 and Bezostaja-01) A THESIS SUBMITTED TO THE GRADUATE SCHOOL OF NATURAL AND APPLIED SCIENCES OF MIDDLE EAST TECHNICAL UNIVERSITY BY MUSA KAVAS IN …
Tissue culture and Agrobacterium-mediated transformation ...
Summary Hemp ( Cannabis sativa L.) is cultivated in many parts of the world for ils fiber, oil, and seed. The development of new hemp cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to investigate the propagation of hemp in tissue culture and to establish a protocol for <i>Agrobacterium</i> …
A novel and rapid method for Agrobacterium-mediated ...
15/10/2021·Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.) In Vitro Cell. Dev. Biol., Plant , 39 ( 2003 ) , pp. 578 - 585 , 10.1079/IVP2003454 View Record in Scopus Google Scholar
Advances and Perspectives in Tissue Culture and ... - MDPI
26/05/2021·Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and …
Full article: Agrobacterium infection of hemp (Cannabis ...
(2013). Agrobacterium infection of hemp (Cannabis sativa L.): establishment of hairy root cultures. Journal of Plant Interactions: Vol. 8, No. 4, pp. 312-320.
Hemp (Cannabis sativa L.) | Springer Nature Experiments
Feeney M, Punja ZK (2003) Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.). In Vitro Cell Dev Biol Plant …
In vitro plant tissue culture as the fifth generation of ...
23/03/2022·Zhang, X. et al. Establishment of an Agrobacterium-mediated genetic transformation and CRISPR/Cas9-mediated targeted mutagenesis in Hemp (Cannabis Sativa L.). Plant Biotechnol. J. 1 , 1–9.
Feeney, M. and Punja, Z.K. (2003) Tissue Culture and ...
Article citations More>>. Feeney, M. and Punja, Z.K. (2003) Tissue Culture and Agrobacterium Mediated Transformation of Hemp (Cannabis sativa L.). In Vitro Cellular & Developmental Biology—Plant, 39, 578-585.
tissue culture and agrobacterium-mediated transformation ...
Tissue culture and agrobacterium-mediated transformation of hemp (cannabis sativa l.) Plant. SummaryHemp (Cannabis sativa L.) is cultivated in many parts of the world for its fiber, oil, and seed. The development of new hemp cultivars with improved traits could be facilitated through the application of biotechnological strategies.
In Vitro Germination and Tissue Culture Protocol ...
Key words: Cannabis; Hemp; Tissue culture; mass propagation, MS medium. 1.INTRODUCTION Cannabis is a genus of flowering plants of the Moraceae family and includes Cannabis indica, Cannabis sativa, and Cannabis ruderalis which exhibit morphological differences like height of the plant, branch number, bushy growth etc. [1]. Most of the …
TISSUE CULTURE AND AGROBACTERIUM-MEDIATED …
01/11/2003·Hemp (Cannabis sativa L.) is cultivated in many parts of the world for its fiber, oil, and seed.The development of new hemp cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to investigate the propagation of hemp in tissue culture and to establish a protocol for Agrobacterium …
A novel and rapid method for Agrobacterium-mediated ...
15/10/2021·Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.) In Vitro Cell. Dev. Biol., Plant , 39 ( 2003 ) , pp. 578 - 585 , 10.1079/IVP2003454 View Record in Scopus Google Scholar
Production of Cannabinoids Using Tissue Culture and ...
Feeney and Punja were the pioneers in cannabis transformation (2003). The agrobacterium mediated transformation resulted in well-developed calli on MS medium with B5 vitamins supplemented with 2, 4 D and KIN. However, the obtained callus did not regenerate. In 2006, Wahby et al used A. rhizogenes for root infection. They identified secondary ...
Professor Zamir Punja – Ensuring Quality ... - .GLOBAL
14/05/2017·Professor Punja’s lab previously demonstrated that in hemp, a combination of the techniques of plant tissue culture and Agrobacterium-mediated genetic transformation can be used to produce genetically-transformed calluses, the first step in producing fully transformed plants of this species. Professor Punja’s work has also focussed on applying transgenic …
Establishment and optimization of a hemp (Cannabis …
26/02/2020·Feeney, M. & Punja, Z. K. Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.). Vitro Cellular & Developmental Biology – Plant. 39 , 578–585 (2003).
Medical Cannabis and Industrial Hemp Tissue Culture ...
03/03/2021·Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.). In Vitro Cell. Dev. Biol. 39, 578–585. doi: 10.1079/IVP2003454 CrossRef Full Text | Google Scholar
Medical Cannabis and Industrial Hemp Tissue Culture ...
03/03/2021·Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.). In Vitro Cell. Dev. Biol. 39, 578–585. doi: 10.1079/IVP2003454 CrossRef Full Text | Google Scholar